Nikon ECLIPSE LV150 optical microscope
The optical microscope in the cleanroom is a Nikon ECLIPSE LV150 optical microscope.
The manufacturers manual can be found here: nikon_lv150-150a_manual.pdf
DO's and DONT's
- DO NOT change the calibration settings!
- DO NOT spill Aceton on the microscope and microscope stage, Aceton can dissolve many plastics
- DO turn off the light when leaving the microscope, the lamp has a finite lifetime
Saving an image with annotations
To save annotations/measurements on an image, first capture the image. Make sure the correct objective is selected at which you took the picture, otherwise the measurements will be off.
Use one of the Annotations and measurement tools to make your measurements. Use the option 'Burn overlay', shortcut key 'Y', to burn the annotation layer into your captured image. Now you can save the image.
Stitching images together, creating a sample overview
With the option 'Scan large image' you can create an overview picture of your complete sample. The microscope starts at the upper left corner of the sample takes a picture, moves the stage automatically and continues to scan the complete sample. Make sure you deselect 'image registration', otherwise the software will likely crash.
To get extremely high resolution images you can do this at high magnifications.