1. If you are new and belong either to the IP or BP group, you should get an “Intro” from the people in charge before using the equipment.
2. If you are new and NOT belong to IP of BP group, please contact T.H. Oosterkamp (oosterkamp@physics.leidenuniv.nl) about using the facilities. Afterwards you can ask for an “Intro”.
3. When you have received an “Intro” and you have shown to be able to carry on your experiments reliably and without damaging the equipment, you will be granted the permission to reserve the equipment.
4. You MUST read the “Safety Regulations” here under before getting access to the Bio SPM Lab facilities.

• Create an account if you don't have one here
• Reserve the equipment (if you don't have permission you will not be able to reserve. If you received an “Intro” you can ask to get the permissions activated)
• If you do not show up after 45 minutes from the start of your booking time, someone else may be allowed to take over your time.
• People who want to do a quick check: get up early. Until 10:30 it's walk-in users only at the wall DI AFM.

• Rule number ZERO is: if you don't know, ASK. Failing to ask something critical may result in expensive equipment damage.
• Scanning Probe Techniques are sensitive to acoustic and mechanical vibrations: do not shout loud, do not make excessive noise, do not jump and smash doors in the Bio SPM labs.
• Scanning Probe Microscopes (all the parts, heads, tip, sample mounts etc) are sensitive to dirt, organic and powder contamination. USE UHV GLOVES when handling all these parts, including when mounting sample and tip!
• Keep the lab tables tidy and clean!
• Fill in the common LOGBOOK EVERY TIME you measure.
• You experience a problem with a setup? Contact the people here a.s.a.p. If you solve it, log it here and talk about it at the Bio SPM Lab meeting.
• You see that some stock (tips, etc) is running out? Order new a.s.a.p. or ask someone to do it for you.
• Done with a measurement? Retract the tip, set X,Y,Z high voltage to ZERO (Scan size, Offsets=0). Remove sample and tip holder. Close the door of the sound box.
• If you use the Bio SPM Lab regularly, you are supposed to attend the Bio SPM Lab on regular basis (every Tuesday at 4 pm, Oort 173).

Everyone working in the Bio SPM Lab should have received the LION safety regulations and have read them thoroughly.
(http://www.physics.leidenuniv.nl/divers/Webversie_mei2005.pdf).
The faculty course about chemical safety should be followed as well as the fire extinguishing drill (follow these at the first opportunity).

You should be able to locate:

• The fire extinguishers in each room
• The emergency off-switches in each room
• Eye showers of each chemical working place
• Emergency showers on each side of the floor
• Point exhausts
• Fire alarm

The general idea is: if you are not sure, ask. Some of the situations you can encounter where you should consider your safety are: chemicals, high voltages, RF radiation, heavy objects, sharp objects etc.

Be very careful to avoid eye contact with all chemicals. If you get strong alkalies in your eye your vision will almost certainly be damaged! Avoid all skin contact with innorganic chemicals (acids and bases) and avoid their vapours by working in a fume hood. Avoid skin contact and especially inhalation of organic chemicals by working in a downflow bench, cleanbench or fumehood (you can find many in the Nanolab, located on the 6th floor. Prior usage, ask to Marcel Hesselberth (hessel@physics.leidenuniv.nl, tel:1887) for permission.)

Always hold a bottle with 2 hands and never even think of just holding the cap. Some organics require evacuation of the building if you drop a bottle! If a bottle breaks and chemicals are spilled there may be an explosion danger. Walk to the 3 button wall panel and switch all 3 buttons off so that the room is completely shut down, then warn the floor coordinator (galli@physics.leidenuniv.nl, tel:5468).

Each cleanbench / downflow bench / fume hood has an eye shower on top. Eye showers are single use! Do not twist the cap out of curiosity, once opened they cannot be closed and must be discarded. If you get covered by chemicals there are showers in the hall on each side of the floor.

MSDS's of all used chemicals are present.

Frequently used chemicals are:

• Acetone
• Ethanol

You can look up the MSDS at http://www.chemexper.com. Many links to MSDS site can be found at http://www.ilpi.com/msds/.

Use demi water for making etch chemicals, NEVER normal water! Demi water is a chemical, do not drink it (it depletes you of minerals).

When working with acids, always wear gloves and work behind the glass of the downflow bench / fume hood or wear safety glasses (available in the 'magazijn'. If you work with HF be extremely careful. HF is not a very strong acid but it is exceptionally destructive. A small area of the body covered with HF is lethal. The only way to neutralize HF is with Ca, it is obligatory to have Ca pills or creme close when you use HF. Be careful when disposing strong acids, strong exothermal reactions can occur upon mixing with other chemicals.

Safety glasses are mandatory! Always! Wear gloves as well. Be careful when disposing strong bases, strong exothermal reactions can occur upon mixing with other chemicals.

There is no type of gloves that protects the user from all known chemical hazards. The two currently available types are Nitrile (blue) and Latex (milky-white). Use the following link to decide which type of gloves you should use to protect your hands from most common chemicals found in clean rooms: http://www.safeskin.com/ChemResist/search.asp?o=1 An exaustive list of all possible chemicals and hazards is here: http://www.pp.okstate.edu/ehs/HAZMAT/gloves5.htm

Both types will protect your sample from your fingerprints; however, it is very important to use
NON-POWDERED gloves (these words should be clearly stated on the box).

Powders have a large surface/volume ratio, this increases the reactivity/toxicity. Heavy metals are toxic, some in small concentrations. Some metals (for example Ce) burn spontaneously in air. Take these considerations into account if using such metals.

Gases under pressure can be very dangerous, work on gas cylinders and lines should only be done by technicians. O₂ at high partial pressures poses fire/explosion danger. Do not pump O₂ with normal rotary pumps. SF₆, Cl, and other etching gases are very toxic. Inert gases are dangerous because they displace air, so breathing Ar is dangerous (First aid: hold victim upside down). Do not breath He to get a funny voice! It can cause lung damage.

Many systems work with very high voltages, and in some cases HV power supplies can deliver significant current (several amps). The systems are equipped with protection circuits but these can never cover all possibilities, so when you disconnect power supply cables to connect them to a different sputtering source for example or when you open vacuum systems be very careful that the mains are switched off.

Also be careful with water leaks in electrical systems and with jewelery (a necklace can dangle through the ventilation slits of an electric box and is therefore not allowed).